Figure 4
From: White-tailed deer are susceptible to the agent of sheep scrapie by intracerebral inoculation
Representative western blot of deer with scrapie. This blot compares brain homogenates from the sheep scrapie inoculum (lane 2), a white-tailed deer with CWD [16] (lane 3), and white-tailed deer with scrapie (deer 5, lane 4-5). All brain homogenates are positive using monoclonal antibodies P4 and 6H4 and tissues with a characteristic 3-band pattern representing diglycosylated, monoglycosylated, and unglycosylated forms of PrPSc. Homogenates derived from cerebrum (lane 5) have a lower migration of the unglycosylated band than those derived from brainstem at the level of obex (lanes 4). Lanes were loaded as follows: sheep scrapie inoculums = 0.1 mg, white-tailed deer CWD = 0.175 mg; deer 5 obex = 1 mg, deer 5 cerebrum = 1 mg, Marker = molecular weight marker.