Representative western blot of deer with scrapie. This blot compares brain homogenates from the sheep scrapie inoculum (lane 2), a white-tailed deer with CWD  (lane 3), and white-tailed deer with scrapie (deer 5, lane 4-5). All brain homogenates are positive using monoclonal antibodies P4 and 6H4 and tissues with a characteristic 3-band pattern representing diglycosylated, monoglycosylated, and unglycosylated forms of PrPSc. Homogenates derived from cerebrum (lane 5) have a lower migration of the unglycosylated band than those derived from brainstem at the level of obex (lanes 4). Lanes were loaded as follows: sheep scrapie inoculums = 0.1 mg, white-tailed deer CWD = 0.175 mg; deer 5 obex = 1 mg, deer 5 cerebrum = 1 mg, Marker = molecular weight marker.