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Table 4 Structural proteins of AngHV-1 as identified by 1D gel and 2D nanoLC-MS/MS

From: Identification and localization of the structural proteins of anguillid herpesvirus 1

ORFa

NCBI ID

Locationb

Descriptionc

Predicted molecular mass (kDa)

1D gel/nanoLC-MS/MSd

2D nanoLC-MS/MSe

     

Number of peptides

Mascot score

EmPAI

Number of peptides

Mascot score

EmPAI

8

282174045

Envelope

Membrane protein type 1

21.2

29

1138

1.3

37

1339

2.59

14

282174051

Tegument

ORF3 family

32.3

-

-

-

-

-

-

16

282174053

Tegument-associated

ORF13 family

33.8

12

349

0.88

11

465

0.66

17

282174054

Tegument-associated

ORF13 family

25.8

21

1010

0.51

10

459

0.70

18

282174055

Tegument

 

233.6

-

-

-

-

-

-

19

282174056

Tegument

 

93.7

5

179

0.17

13

713

0.30

20

282174057

Tegument

 

63.1

3

123

0.19

-

-

-

24

282174061

Tegument-associated

ORF13 family

29.3

11

493

1.07

11

471

0.59

26

282174063

Tegument-associated

 

18.4

-

-

-

-

-

-

30

282174067

Tegument-associated

 

118.4

23

801

0.54

16

450

0.34

32

282174069

Tegument-associated

 

20.0

5

239

1.41

-

-

-

34

282174071

Tegument-associated

 

195.0

71

2641

1.32

58

2200

0.71

35

282174072

Tegument-associated

 

33.4

19

797

1.35

18

632

0.67

36

282174073

Capsid

Capsid triplex protein 2

40.2

29

1016

3.99

22

856

1.80

38

282174075

Tegument

 

44.1

4

142

0.39

-

-

-

39

282174076

Tegument

 

62.7

2

57

0.12

-

-

-

40

282174077

Tegument

 

162.7

22

783

0.56

13

446

0.29

42

282174079

Capsid

Capsid triplex protein 1

42.9

20

979

1.73

17

558

1.42

43

282174080

Tegument-associated

 

18.0

4

121

0.48

8

291

1.12

48

282174085

Capsid

 

102.6

32

1112

1.18

26

876

0.97

49

282174086

Envelopef

Membrane protein type 3

26.4

1

38

0.14

1

42

0.14

51

282174088

Envelope

Major envelope protein/Membrane protein type 3

26.5

30

1042

4.70

30

972

2.18

57

282174094

Capsid

Capsid protease-and-scaffolding protein

78.0

9

345

0.45

8

328

0.43

66

282174103

Envelope

Membrane protein type 1

42.7

6

458

0.18

-

-

-

67

282174104

Envelope

WBV spike protein/M ajor glycoprotein/Membrane protein type 1

152.9

25

948

0.43

15

577

0.26

71

282174108

Envelope

Membrane protein type 1

11.4

4

172

0.82

3

103

0.33

78

282174115

Envelope

Membrane protein type 3

16.9

4

146

0.23

-

-

-

81

282174118

Tegument-associated

 

51.5

7

226

0.63

1

56

0.07

83

282174120

Tegument-associated

Cysteine protease domain/Large tegument protein

376.8

166

6652

1.46

104

3870

0.71

95

282174132

Envelope

ISAV HA/Membrane protein type 1

41.6

54

2069

1.81

52

2256

1.48

100

282174137

Capsid

 

61.1

2

66

0.13

1

33

0.03

103

282174140

Tegument

  

-

-

-

-

-

-

104

282174141

Capsid

Major capsid protein

139.9

213

8402

5.33

104

4058

2.56

108

282174145

Envelope

ORF80 family

108.2

-

-

-

1

33

0.06

114

282174151

Tegument-associated

 

20.7

28

852

3.64

13

550

1.27

115

282174152

Envelope

  

-

-

-

-

-

-

125

282174162

Envelope

ORF109 family

 

-

-

-

-

-

-

126

282174163

Capsid

 

22.4

1

30

0.17

-

-

-

128

282174165

Tegument-associated

 

17.9

4

139

0.48

1

60

0.21

129

282174166

Tegument-associated

 

42.2

8

290

0.19

6

238

0.18

  1. athe ORF are ordered by number with the ORF only detected in the virion fractions presented in italics, blocation as determined in this paper, cproperties newly suggested in this paper are presented in italics, dnumber of peptides, Mascot score and emPAI in the complete virion fraction as determined by 1D gel/nanoLC-MS/MS, enumber of peptides, Mascot score and emPAI in the complete virion fraction as determined by 2D nanoLC-MS/MS, fAngHV-1 ORF49 was not detected in the envelope fraction and classified as an envelope protein only on the basis of predicted structural properties.
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Veterinary Research

ISSN: 1297-9716