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Table 4 Structural proteins of AngHV-1 as identified by 1D gel and 2D nanoLC-MS/MS

From: Identification and localization of the structural proteins of anguillid herpesvirus 1

ORFa NCBI ID Locationb Descriptionc Predicted molecular mass (kDa) 1D gel/nanoLC-MS/MSd 2D nanoLC-MS/MSe
      Number of peptides Mascot score EmPAI Number of peptides Mascot score EmPAI
8 282174045 Envelope Membrane protein type 1 21.2 29 1138 1.3 37 1339 2.59
14 282174051 Tegument ORF3 family 32.3 - - - - - -
16 282174053 Tegument-associated ORF13 family 33.8 12 349 0.88 11 465 0.66
17 282174054 Tegument-associated ORF13 family 25.8 21 1010 0.51 10 459 0.70
18 282174055 Tegument   233.6 - - - - - -
19 282174056 Tegument   93.7 5 179 0.17 13 713 0.30
20 282174057 Tegument   63.1 3 123 0.19 - - -
24 282174061 Tegument-associated ORF13 family 29.3 11 493 1.07 11 471 0.59
26 282174063 Tegument-associated   18.4 - - - - - -
30 282174067 Tegument-associated   118.4 23 801 0.54 16 450 0.34
32 282174069 Tegument-associated   20.0 5 239 1.41 - - -
34 282174071 Tegument-associated   195.0 71 2641 1.32 58 2200 0.71
35 282174072 Tegument-associated   33.4 19 797 1.35 18 632 0.67
36 282174073 Capsid Capsid triplex protein 2 40.2 29 1016 3.99 22 856 1.80
38 282174075 Tegument   44.1 4 142 0.39 - - -
39 282174076 Tegument   62.7 2 57 0.12 - - -
40 282174077 Tegument   162.7 22 783 0.56 13 446 0.29
42 282174079 Capsid Capsid triplex protein 1 42.9 20 979 1.73 17 558 1.42
43 282174080 Tegument-associated   18.0 4 121 0.48 8 291 1.12
48 282174085 Capsid   102.6 32 1112 1.18 26 876 0.97
49 282174086 Envelopef Membrane protein type 3 26.4 1 38 0.14 1 42 0.14
51 282174088 Envelope Major envelope protein/Membrane protein type 3 26.5 30 1042 4.70 30 972 2.18
57 282174094 Capsid Capsid protease-and-scaffolding protein 78.0 9 345 0.45 8 328 0.43
66 282174103 Envelope Membrane protein type 1 42.7 6 458 0.18 - - -
67 282174104 Envelope WBV spike protein/M ajor glycoprotein/Membrane protein type 1 152.9 25 948 0.43 15 577 0.26
71 282174108 Envelope Membrane protein type 1 11.4 4 172 0.82 3 103 0.33
78 282174115 Envelope Membrane protein type 3 16.9 4 146 0.23 - - -
81 282174118 Tegument-associated   51.5 7 226 0.63 1 56 0.07
83 282174120 Tegument-associated Cysteine protease domain/Large tegument protein 376.8 166 6652 1.46 104 3870 0.71
95 282174132 Envelope ISAV HA/Membrane protein type 1 41.6 54 2069 1.81 52 2256 1.48
100 282174137 Capsid   61.1 2 66 0.13 1 33 0.03
103 282174140 Tegument    - - - - - -
104 282174141 Capsid Major capsid protein 139.9 213 8402 5.33 104 4058 2.56
108 282174145 Envelope ORF80 family 108.2 - - - 1 33 0.06
114 282174151 Tegument-associated   20.7 28 852 3.64 13 550 1.27
115 282174152 Envelope    - - - - - -
125 282174162 Envelope ORF109 family   - - - - - -
126 282174163 Capsid   22.4 1 30 0.17 - - -
128 282174165 Tegument-associated   17.9 4 139 0.48 1 60 0.21
129 282174166 Tegument-associated   42.2 8 290 0.19 6 238 0.18
  1. athe ORF are ordered by number with the ORF only detected in the virion fractions presented in italics, blocation as determined in this paper, cproperties newly suggested in this paper are presented in italics, dnumber of peptides, Mascot score and emPAI in the complete virion fraction as determined by 1D gel/nanoLC-MS/MS, enumber of peptides, Mascot score and emPAI in the complete virion fraction as determined by 2D nanoLC-MS/MS, fAngHV-1 ORF49 was not detected in the envelope fraction and classified as an envelope protein only on the basis of predicted structural properties.