Figure 1

Discriminatory WB of Greek scrapie positive goats. WB of proteinase K-treated PrP^res from the obex of Greek goats (GR-A4-G2, GR-E1-G1, GR-A4-G3 and GR-B1-G1 loaded in lanes 2-4 and 6, respectively), an Italian goat (IT1-goat, lane 7), a sheep BSE (lane 8) and an Italian sheep used as internal control (IT5-sheep, lane 9). PrP^res was detected by mAbs SAF84 (panel A) or P4 (panel B) using molecular markers (lanes 1, 5, 10). Note that sample GR-E1-G1 (lane 3) is negative with SAF84 and positive with P4 and this is probably due to P168Q polymorphism within the SAF84 epitope.