Occludin and collagen IV degradation mediated by the T9SS effector SspA contributes to blood–brain barrier damage in ducks during Riemerella anatipestifer infection

Table 2 Samples used for different experiments

Time point	Sample characteristics			Experiments
24 h	28	Divided into four parts	28	Bacterial enumeration
			28	Gene and protein verification
			12	Haematoxylin–eosin staining
			0	TEM observation
	12	Divided into two parts	12	Bacterial enumeration
	12	Divided into two parts	12	BBB permeability assay
48 h	28	Divided into four parts	28	Bacterial enumeration
			28	Gene and protein verification
			12	Haematoxylin–eosin staining
			12	TEM observation
	12	Divided into two parts	12	Bacterial enumeration
	12	Divided into two parts	12	BBB permeability assay

ISSN: 1297-9716