Table 1 Primers used for gene amplification
Gene product (source) | Sequences | Product length (bp) |
|---|---|---|
ZO-1 (duck) | F: 5ʹ-CCAGTAGCTCGACAGGAACC-3′ | 146 |
R: 5ʹ-CTTGCGCTTCGGTCTCTTAC-3′ | ||
Occludin (duck) | F: 5′-AATTCGACACCGACCTCAAG-3′ | 254 |
R: 5′-CTTGTCGTAGTCGCTCACCA-3′ | ||
Laminin-α4 (duck) | F:5′-TGCACACAACCTACGAGGAC-3′ | 188 |
R: 5′-CAGCAGCATCATTGACACGG-3′ | ||
IL-β1 (duck) | F:5′-TCATCTTCTACCGCCTGGAC-3′ | 244 |
R: 5′-GTAGGTGGCGATGTTGACCT-3′ | ||
IL-6 (duck) | F:5′-TTCGACGAGGAGAAATGCTT-3′ | 150 |
R: 5′-CCTTATCGTCGTTGCCAGAT-3′ | ||
IL-8 (duck) | F:5′-TGGTAAGGATGGGAAACGAG-3′ | 211 |
R: 5′-TTGGCCAGAATTGCCTTTAC-3′ | ||
IL-10 (duck) | F:5′-CTGACCTCCTACCAGCGAAG-3′ | 179 |
R: 5′-CTCCATGTAGAACCGCATCA-3′ | ||
IL-17A (duck) | F:5′-TGCCTACGGGAAGGTGATAC-3′ | 211 |
R: 5′-GTGGTCCTCATCGATCCTGT-3′ | ||
IL-17RA (duck) | F:5′-CAAGTCCGCATCTCTCACAA-3′ | 171 |
R: 5′-ATTTGGTACCGCGTTGACTC-3′ | ||
CCL3 (duck) | F:5′-CTTTGCTACGAGAACCTGGC-3′ | 249 |
R: 5′-CTAGCTGGGGATGGAGAAGG-3′ | ||
CCL4 (duck) | F:5′-GCTCTTCTCCTCATTGCAGC-3′ | 212 |
R: 5′-CAGGTGTCACTTGGATTGGC-3′ | ||
CCL5 (duck) | F:5′-CTGTGGCCCATCTTGACAAC-3′ | 202 |
R: 5′-TCAAAGTGCTTCAGGTGTGC-3′ | ||
CCR5 (duck) | F:5′-TGGGCCTACTATGCTGTTCA-3′ | 230 |
R: 5′-GGAACAGAGGCAAGCATAGC-3′ | ||
CX3CL1 (duck) | F:5′-AGCAGCAGTTCCAGAAAAGC-3′ | 162 |
R: 5′-TTGCTGGTCACCTCTGTCAT-3′ | ||
TNFSF13β (duck) | F:5′-TGATGACGAGAAAGCAGGTG-3′ | 189 |
R: 5′-AAGCAGGCCTGTAGCACTGT-3′ | ||
ITGβ2 (duck) | F:5′-AATTCAGGCTACACGGGGAA-3′ | 175 |
R: 5′-CGTAGATCTGCTTGTTGGGC-3′ | ||
PECAM-1 (duck) | F:5′-GTGGGACTGCTTTTCTCTGC-3′ | 212 |
R: 5′-TGGATGGCTTTTCACATTCA-3′ | ||
VCAM-1 (duck) | F:5′-TTCCTTCTGATCGCCTGGAA-3′ | 239 |
R: 5′-TGGGCCAAAGTTTGCATTCA-3′ | ||
SELE (duck) | F:5′-CCGCAACCATAACGCTAAGT-3′ | 206 |
R: 5′-CCTCATTTCCATTGCCATTT-3′ | ||
SELP (duck) | F:5′-CTGCCGCCTTCTTAAAGCAA-3′ | 177 |
R: 5′-TGGTGAAGATGCCTGGTGAT-3′ | ||
β-Actin (duck) | F:5′-GAGAAATTGTGCGTGACATCA-3′ | 153 |
R: 5′-CCTGAACCTCTCATTGCCA-3′ | ||
Occludin (duck) | F:5′-TAGTCCAGTGTGGTGGAATTATGTTCAGCAAGAAGTCCTA-3′ | 1524 |
R: 5′-GCTGGATATCTGCAGAATTCACCCCGCACCTTGTCGTAG-3′ | ||
sspA (R. anatipestifer) | F:5′-TGAACCGTCAGAATTAAGCTTATGAAGAAGATCATCTTTGT-3′ | 2130 |
R: 5′-GAATTCGCGGCCGCAAGCTTCTTCTTGATGAACTTCTTAG-3′ | ||
Claudin-5 (bEnd.3) | F: 5′-CGCTTGTGGCACTCTTTGT-3′ | 168 |
R: 5′-ACTCCCGGACTACGATGTTG-3′ | ||
JAM2 (bEnd.3) | F: 5′-CTAGTGGCTCCTGCTGTTCC-3′ | 108 |
R: 5′-GTACTCCGGAGCTGGGTTTC-3′ | ||
VCAM-1 (bEnd.3) | F: 5′-ATTTTCTGGGGCAGGAAGTT-3′ | 179 |
R:5′-ACGTCAGAACAACCGAATCC-3′ | ||
SELE (bEnd.3) | F: 5′-ACACATCTGGTGGCAATTCA-3′ | 211 |
R:5′-TGTTGTTTGGTTCACCTGGA-3′ | ||
β-Actin (bEnd.3) | F: 5′-AGCCATGTACGTAGCCATCC-3′ | 228 |
R:5′-CTCTCAGCTGTGGTGGTGAA-3′ |