Figure 3

SspA affects the expression of TJPs, BM proteins and CAMs in the brain. A Changes in the expression of these molecules at the transcriptional level. mRNA expression was measured by qPCR, and relative expression was calculated using the 2^−ΔΔCt method. Compared with those in animals infected with the wild-type strain Yb2, the mRNA levels of ITG-β2, PECAM-1, VCAM-1, SELE, and SELP were significantly lower and the levels of ZO-1, occludin, and laminin-α were significantly greater in animals infected with the SspA deletion mutant strain Yb2ΔsspA. B Western blot analysis of protein expression using the Quantity One program. Duck β-actin was used as a normalization control in the analyses. The data are presented as the means ± standard deviations of three animals from triplicate experiments. Statistical significance was assessed using one-way analysis of variance (ANOVA). The asterisks indicate the level of statistical significance (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).