Figure 1

SADS-CoV infection upregulates TRIM25 expression. A and C Relative expression levels of TRIM25 were quantified by qRT-PCR. IPI-2I cells were either mock infected or infected with SADS-CoV 0.1 MOI at different times (A) or different MOIs at 24 hpi (C). Total RNA was extracted, and TRIM25 mRNA levels were evaluated with qRT-PCR. B and D Relative expression levels of TRIM25 were quantified by Western blotting. IPI-2I cells were either mock infected or infected with SADS-CoV 0.1 MOI at different times (B) or different MOIs at 24 hpi (D). The cell lysates were collected, and the expression of TRIM25 was tested by Western blotting. The densities of TRIM25 bands relative to GAPDH were calculated. The value of each negative control cells group was normalized to one. E Representative microphotographs of viral antigen immunochemical staining in SADS-CoV -non-infected and -infected ileum tissues (Bar: 50 µm). F-I SADS-CoV infection upregulates TRIM25 expression in vivo. F and G Western blotting analysis of the protein levels of TRIM25 in ileum (F) and jejunum (G) samples from SADS-CoV -non-infected and -infected piglets at 36 hpi. The densities of TRIM25 bands relative to GAPDH were calculated. The value of control piglet was normalized to one. H and I qRT-PCR analysis of the mRNA expression levels of TRIM25 in ileum (H) and jejunum (I) samples from SADS-CoV -non-infected and -infected piglets at 36 hpi. P values were calculated using two-tailed unpaired Student’s t-test.