Figure 2From: STING-dependent trained immunity contributes to host defense against Clostridium perfringens infection via mTOR signalingTrained immunity activates STING signaling. WT PM were trained with HKCA and then were restimulated with LPS or C. perfringens at an MOI of 5 for the indicated times. A, D Whole cell lysates were analyzed for STING, p-TBK1, p-IRF3, and β-actin by Western blotting. B, C, E, and F The gray intensity value of proteins was calculated using ImageJ software. G IFN-β mRNA levels were measured at 4 h post‑infection by qRT‑PCR. H CXCL10 mRNA levels were measured at 24 h post‑infection by qRT‑PCR. Data are shown as mean ± SEM. Data were pooled from 3 independent experiments. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.Back to article page