A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection

Table 2 Primers and probes used in this study for constructing infectious clones and detecting viral loads

Primers/Probes	Sequence (5 → 3)^a	Length (bp)
HLJB1-SgsI-F1	AGCTCGGGCGCGCCTACATGATGTGTAGGGTATT	34
HLJB1-Pfl23II-R1	ATGGCCTGACTAGGATCGTACGGAACGGCAACCACCGT	38
HLJB1-Pfl23II-F2	ACGGTGGTTGCCGTTCCGTACGATCCTAGTCAGGCCAT	38
HLJB1-BglII-R2	AGCACAGCATCGAGAGGAGATCTACCAGCCCCAACCGGT	39
HLJB1-BglII-F3	ACCGGTTGGGGCTGGTAGATCTCCTCTCGATGCTGTGCT	39
HLJB1-Bsp1407I-R3	ATAAGCAAGTCCGCGTTGTACAAATACGATTCATCGGT	38
HLJB1-Bsp1407I-F4	ACCGATGAATCGTATTTGTACAACGCGGACTTGCTTAT	38
1R4	*AGCGAGGAGGCTGGGACCAT*GCCGGCCTTTTTTTTTTTTTTTTTTTTTAATTTCGGTCACATGGTTCCT	69
XbaI-2R4	ACAGTCTAGAGTCCCATTCGCCATTACCGAGGGGACGGTCCCCTCGGAATGTTGCCCAGCCGGCGCCAGCGAGGAGGCTGGGACCAT^b	87
PRRSV1-ORF2-CON-R	acagtgaccccattgcatcgctccgagttcac^c	32
PRRSV1-ORF2-CON-F	gtgaactcggagcgatgcaatggggtcactgt	32
PRRSV1-ORF6-CON-R	cttctggctctgatttttaccggccatacttgacg	35
PRRSV1-ORF7-CON-F	cgtcaagtatggccggtaaaaatcagagccagaag	35
PRRSV1-Fusion-R	atggctggcaactagaaggcacag	24
PRRSV1-UF	cagatgcagattgtgttgcct	20
PRRSV1-UR	atggagacctgcagcactttc	20
PRRSV1-UP	FAM-tctggcccctgccca-MGB	14
SD1291-qPCR-F	agatgctgggcgcaatgata	20
SD1291-qPCR-R	ggcttctcaggctttttccttta	24
SD1291-qPCR-P	FAM-ggcggcccagggg-MGB	13

^aThe unique restriction enzymes used for cloning are underlined.
^bThe hepatitis D virus ribozyme sequence is shown in italics, and the overlapping regions are marked in bold.
^cThe primers and probes used for PRRSV1 ORF2-6-CON construction and PRRSV1 detection are shown in lowercase.

ISSN: 1297-9716