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Figure 2 | Veterinary Research

Figure 2

From: A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection

Figure 2

Dynamics of viral load, antibody level, rectal temperature and weight gain during animal inoculation and challenge study. A Viremia was detected via a PRRSV1 real-time RT‒PCR assay [31]. B PRRSV-specific antibodies were detected with an IDEXX HerdCheck PRRS × 3 ELISA Kit. C Rectal temperature was determined daily for 14 dpi after inoculation and challenge and weekly thereafter. D Body weight was recorded weekly for all groups of pigs. E SD1291 viral loads in the lungs, lymph nodes and tonsils among the distinct groups were determined using the SD1291 strain-specific primers and probes shown in Table 2. F The gross pathology of the lungs was scored according to methods described previously [33,34,35]. The data shown in the bar graphs are the means and SDs. Statistical significance is denoted by *, p < 0.05; **, p < 0.01; and ***, p < 0.001 (n = 5).

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Veterinary Research

ISSN: 1297-9716