Figure 2From: Comparative analysis of the interactions of different Streptococcus suis strains with monocytes, granulocytes and the complement system in porcine bloodS. suis strains show differences in the level of association with monocytes and lymphocytes. Porcine peripheral blood mononuclear cells (PBMCs) freshly isolated from porcine blood were incubated with Far Red-labeled S. suis strains (S. suis*FR) at an MOI of 1 for 30 min at 37 ℃, whereby S. suis*FR had been pre-incubated in serum of colostrum-deprived piglets (CDS) for 30 min (cps2, cps14, cps9, and cps7). Monocytes were stained using the myeloid marker CD172a-FITCs and samples were measured by flow cytometry. The levels of associations of S. suis strains with monocytes (A–C) and lymphocytes (D–F) are shown. Each symbol refers to a different piglet. Data shown in one graph was obtained in one experiment. Horizontal lines and error bars represent mean values and SDs. For statistical analysis, paired t-test (cps2 strains 10 vs 483) or one-way analyses of variance (ANOVAs) with Tukey’s multiple comparisons test (cps2 strain 10 vs cps14 strains or cps2 strains vs cps9 vs cps7 strains) were performed. Significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001).Back to article page