Primer | Sequence (5′-3′a) | Usage |
---|---|---|
pGL3-5'UTR-F | cgggctcgagatctATGACGTATAGGTGTTGGCTCTATGC | Amplification of PRRSV 5'UTR |
pGL3-5'UTR-R | cggaatgccaagcttGGTTAAAGGGGTGGAGAGACC | |
pGL3-5'-3'UTR-F | aagatcgccgtgtaaTGGGCTGGCATTCTTTGG | Amplification of PRRSV 3'UTR |
pGL3-5'-3'UTR-R | tcggtcgacggatccTTTTTTAATTGCGGCCGCATGGT | |
pGL3-3'UTR-R | cgccccgactctagaTTTTTTAATTGCGGCCGCATGG | |
q-mZNF283-F | TCCATGGCATAACTTGGGGAA | qRT-PCR for detection of Marc-145 ZNF283 |
q-mZNF283-R | TGGCCATGCATTACACAAACTG | |
q-sZNF283-F | CGAGTTTGCCTGAGAAGAGCG | qRT-PCR for detection of PAM ZNF283 |
q-sZNF283-R | GAAGACACGGGAAAGGGACG | |
q-mGAPDH-F | TGATGACATCAAGAAGGTGGTGAAG | qRT-PCR for detection of Marc-145 GAPDH |
q-mGAPDH-R | TCCTTGGAGGCCATGTGGGCCAT | |
q-sGAPDH-F | CCTTCCGTGTCCCTACTGCCAAC | qRT-PCR for detection of PAM GAPDH |
q-sGAPDH-R | GACGCCTGCTTCACCACCTTCT |