Figure 8From: ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10The KRAB domain of ZNF283 interacts with the aa 178–449 region of Nsp9. A Marc-145 cells were cotransfected with Flag-tagged truncated Nsp9 spanning aa 178–449 and EGFP-tagged ZNF283 KRAB domain spanning aa 56–97 or an EGFP-tagged vector. After 24 h, the cells were stained with DAPI and incubated with antibodies specific for the Flag-tagged protein. B HEK-293T cells were cotransfected with Flag-tagged truncated Nsp9 (aa 178–449) and EGFP-tagged ZNF283 aa 56–97. Thirty-six hours after transfection, the cell lysate was immunoprecipitated with mouse anti-Flag or mouse anti-GFP antibodies and then immunoblotted using the indicated antibodies from different species. C Schematic representation of the interaction between ZNF283 and PRRSV Nsp9.Back to article page