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Figure 5 | Veterinary Research

Figure 5

From: ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

Figure 5

ZNF283 interacts with PRRSV Nsp9 and Nsp10. A Marc-145 cells were cotransfected with HA-tagged ZNF283 and plasmid-encoded Flag-tagged PRRSV viral proteins (Nsp9, Nsp10, Nsp11, and Nsp12) for 24 h. The cells were then fixed, and immunofluorescence analysis was performed using rabbit anti-HA and mouse anti-Flag antibodies. Nuclei were stained with DAPI. B and C HEK-293T cells were cotransfected with HA-tagged ZNF283 and Flag-tagged Nsp9 or Nsp10 for 36 h, followed by immunoprecipitation with mouse anti-Flag or mouse anti-HA antibodies. Cell lysates were analysed by Western blotting using rabbit anti-HA or rabbit anti-Flag antibodies.

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Veterinary Research

ISSN: 1297-9716