Figure 6
From: Characterization of translocon proteins in the type III secretion system of Lawsonia intracellularis
Expression of LI1159 is toxic to E. coli. A Growth curves of E. coli harboring different plasmid constructs under inducing or non-inducing conditions. Expression of LI1158-HA and LI1159-HA was induced by the addition of l-arabinose (+ l-arabinose, red mark). The mean of three independent replicates was used to create the growth curves by GraphPad Prism 7.0. B LI1158/LI1159-HA was expressed upon induction in E. coli cells. Induction of LI1158/LI1159-HA expressions were verified by Western blotting using an anti-HA antibody. C The toxic effect of LI1159 expression in E. coli was confirmed in serial dilution patch tests of E. coli transformants shown in A. Liquid cultures were grown for equal time periods and expression of proteins was induced by the addition of l-arabinose for 1 h. Ten microgram samples were taken and diluted ranging from 10–1 to 10–4. Dilutions were spotted onto solid LB-media with 1% glucose (repressing condition) and incubated overnight at 37°C.