Figure 5

CATH-1 inhibits SS2-induced inflammatory response via a TLR2/4-dependent manner. Macrophages from WT, TLR2^−/− and TLR4^−/− mice were uninfected and infected with SS2 strain SC19 at a MOI of 5 for 2, 4, and 6 h. Then, gentamicin (250 μg/mL) was added and incubated for 22, 20, and 18 h. After 24 h infection, the supernatants were collected to determine the levels of IL-1β, IL-6, and TNF-α in the supernatants (A and B). WT macrophages were incubated with or without CATH-1, CATH-2, CRAMP, and PAMP-36 for 2 h (pre-incubation). Next, cells were washed with Opti-MEM twice and then infected with SC19 at a MOI of 5 for 0, 15, 30, 60, and 120 min to determine TLR2 and TLR4 protein expression (C and D). Data are represented as mean ± SEM of three independent experiments of triplicate samples per experiment. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, ns, no significance.