TurboID screening of ApxI toxin interactants identifies host proteins involved in Actinobacillus pleuropneumoniae-induced apoptosis of immortalized porcine alveolar macrophages

Table 1 The primers used to construct plasmids.

Primers	Sequence	Characteristics
P1	CTAGCTAGCGGTGGCAGCGGTGGCAGCATGAAAGACAATACTGTGCC	To amplify the TurboID fragment
P2	GCTACTAGTGTTTTTTTCATGCTGCCACCGCTGCCACCCTTTTCGGCAGACCGCAGAC	To amplify the TurboID fragment
P3	GGAATTCCATAATGGGTGGCAGCGGTGGCAGCATGGCTAACTCTCAGCTCG	To amplify the ApxIA fragment
P4	CCGCTCGAGAGCTGCTTGTGCTAAAGAATAACTCAAAGAA	To amplify the ApxIA fragment
P5	CTAGCTAGCGGTGGCAGCGGTGGCAGCATGAAAGACAATACTGTGCC	To amplify the His- TurboID-fragment
P6	CCGCTCGAGCTTTTCGGCAGACCGCAGACTG	To amplify the His- TurboID-fragment
P7	GGAATTCCATAATGGGTGGCAGCGGTGGCAGCATGGCTAACTCTCAGCTCG	To amplify the HA-His- ApxIA fragment
P8	CCGCTCGAGAGCTGCTTGTGCTAAAGAATAACTCAAAGAA	To amplify the HA-His- ApxIA fragment
P9	CCGGAATTCGGTGGCAGCGGTGGCAGCATGTCGGGGGGCAAATACGTAG	To amplify CAV1 gene
P10	CCGCTCGAGTTATATTTCTTTCTGCATG	To amplify CAV1 gene
P11	CGCCATATGGGTGGCAGCGGTGGCAGCGCTGCAACCGGCTCATTAG	To amplify the HA-His-ApxI-M1 fragment
P12	CCGCTCGAGAGCTGCTTGTGCTAAAGAATAAC	To amplify the HA-His-ApxI-M1, HA-His-ApxI-M2, and HA-His-ApxI-M3 fragment
P13	CGCCATATGGGTGGCAGCGGTGGCAGCGGACAAAGTGCACAGAAAGC	To amplify the HA-His-ApxI-M2 fragment
P14	CGCCATATGGGTGGCAGCGGTGGCAGCAACTTATTTGACGGTGGTGTAG	To amplify the HA-His-ApxI-M3 fragment
P15	CGCCATATGGGTGGCAGCGGTGGCAGCATGGCTAACTCTCAGCTCGA	To amplify the HA-His-ApxI-M4 fragment
P16	CCGCTCGAGGTCAAATGTTAAGTAACCTGTATCGG	To amplify the HA-His-ApxI-M4 fragment

ISSN: 1297-9716