Figure 5

CAV1 is involved in ApxI-induced apoptosis of iPAM cells. A Expression plasmid transfection and western blot to assess the protein level of CAV1. Corresponding expression plasmids were transfected into iPAM cells for 24 h. Lysates were separated by SDS‒PAGE and detected by western blotting with an antibody against Flag. Western blot detection of the cleaved caspase 3 band (17 kDa) with a caspase 3 antibody. The average intensity of active caspase 3 in the immunoblot was quantified and normalized to the intensity of β-actin. B The effect of CAV1 gene overexpression on apoptosis involving ApxI toxin was examined using the Annexin-V-FITC Apoptosis Detection Kit under fluorescence microscopy. iPAM cells transfected with or without the CAV1 gene were exposed to 50 μg/mL ApxI toxin for 34 h. Graphs show the mean cell apoptosis for each group. A t test showed highly significant changes between the control and overexpression groups (p = 0.0005) (p < 0.001).