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Figure 3 | Veterinary Research

Figure 3

From: TurboID screening of ApxI toxin interactants identifies host proteins involved in Actinobacillus pleuropneumoniae-induced apoptosis of immortalized porcine alveolar macrophages

Figure 3

Validation of interactions between ApxIA protein and host cell proteins with coimmunoprecipitation analysis. A Western blot analysis of Flag-CAV1 plasmids transfected into HEK 293 T cells. Western blot analysis of pet-28a-HA-His-ApxIA transformed into E.coli BL21(DE3). B Immunoblot of Flag-CAV1 recombinant proteins from transfected HEK 293 T cells and HA-ApxIA protein using anti-HA mAb. C Immunoblot of HA-ApxIA protein and host cell proteins precipitated using anti-Flag mAb from HEK 293 T cells transfected with pCAGGS-Flag-CAV1.

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Veterinary Research

ISSN: 1297-9716