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Figure 1 | Veterinary Research

Figure 1

From: TurboID screening of ApxI toxin interactants identifies host proteins involved in Actinobacillus pleuropneumoniae-induced apoptosis of immortalized porcine alveolar macrophages

Figure 1

Expression of His-TurboID-ApxIA and His-TurboID and validation of their efficiency of biotinylation. A A schematic drawing of the His-TurboID-ApxIA and His-TurboID constructs. The ApxIA gene was ligated to the 3′ end of TurboID with a linker encoding a short peptide (GGSGGS). His tag sequence was added to the 5′ end of TurboID for detection and purification of the recombinant proteins. B Analysis of His-TurboID-ApxIA and His-TurboID recombinant protein expression by SDS‒PAGE. C Analysis of His-TurboID-ApxIA and His-TurboID recombinant protein expression by western blot. D Western blot analysis of His-TurboID-ApxIA- and His-TurboID-treated iPAM cells. iPAM cells were treated with His-TurboID-ApxIA, His-TurboID, or untreated in medium with and without supplementation with 50 μM biotin. Total cell lysates were detected by western blot probed with horseradish peroxidase (HRP)-coupled streptavidin.

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Veterinary Research

ISSN: 1297-9716