Figure 4

Neutrophils after 3 h of incubation with live Map at a 1:10 MOI. Neutrophils often swarm around bacteria (inset B, arrow) and release myeloperoxidase (MPO) positive granules on bacterial clumps (inset A). Neutrophils with apparently internalized bacteria are frequent (insets B and C, arrowheads). In this micrograph, several NETs released by small numbers of neutrophils in contact with Map can also be observed, as well as a great number of free MPO + granules. DNA (blue), Map-GFP (green), histones (red), MPO (yellow). Micrograph was taken at 200 × using the Eclipse Ni-E (Nikon) microscope and the Prime BSI Scientific CMOS scientific camera (Photometrics® Prime BSI™).