Figure 2

Immunofluorescence staining of neutrophils after 3 h incubation. Non-infected, unstimulated neutrophils (Control), with Zymosan, PMA (phorbol myristate acetate), heat-inactivated Map (kMAP) and live Map (MAP) at 1:1 and 1:10 MOI. DNA (blue), Map-GFP (green), histones (red), myeloperoxidase (yellow). Micrographs were taken at 400x using the Eclipse Ni-E (Nikon) microscope and the Prime BSI Scientific CMOS scientific camera (Photometrics® Prime BSI™). Representative fields (n = 3, two technical replicates per animal).