Figure 7From: Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread The UL49 gene affects accumulation of viral mRNAs. DEF cells were infected with recombinant viruses at an MOI of 1. Cells were harvested at 48 hpi, RNA was extracted from cells and RT-qPCR was used to detect mRNA levels. The relative gene expression levels were determined using the 2−ΔCt method.Back to article page