Figure 2

Rescue and identification of recombinant viruses. A RFLP analysis. The extracted plasmids were cut with restriction enzyme Hind III or EcoR I (The left diagram shows the expected enzyme digestion results, and the right diagram shows the experimental enzyme digestion results). B The rescue of UL49 mutant virus. The extracted positive clone plasmid was transfected into DEF cells. The expression of the recombinant viral fluorescence-labeled protein EGFP was observed in DEF cells over time. C Western blot analysis of UL49 gene deletion recombinant virus protein expression. D Identification of recombinant viruses by PCR.