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Figure 1 | Veterinary Research

Figure 1

From: Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread

Figure 1

 A schematic diagram of the construction of the BAC-CHv-ΔUL49 virus on the BAC platform. (I) The genome structure of DPV CHv. (II) The first Red recombination, the UL49 gene was replaced by the Kan resistance gene. (III) The product after the first Red recombination. (IV) The second Red recombination, the I- SecI cleavage site was recognized by the recombinase, and the Kan fragment was removed. (V) The genome structure of BAC-CHv-ΔUL49.

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