Table 1 Primers used in this study.
Gene name | Forward | Reverse |
|---|---|---|
Actb | CCAGACATCAGGGTGTGATGG | CTCCATATCATCCCAGTTGGTGA |
Agr2 | CGCAGACGTATGAGGAAGCC | GGTTTCGTACACAAGGTTCAGG |
Bmi1 | CTGCTCAACATCAGGTCAGATA | TCTTCGTCAGCCACTTCTCCC |
Ccl20 | GGCACAAAGCAACCAAGATT | GGATTTACGCAGGCTTTCAG |
CD3d | GACGCTCCCACCATATCAGG | ATCATTCCGCTCACCAAGGG |
CD8a | CGAGGGGTCAAAGCAAG | TGTGGCTGGGAAGAAGG |
CD24 | AGCAAGTTCCACTTTGCCAGC | TTTCCATGTCCATGAGCGGTG |
CD44 | ACGAGGAGCAAAGCATGTGA | GTGAGCCGTCCTCATTGTCA |
Chga | ATCTCCCTTCCTGTGACAAATG | GATCGGCAGTGGGTCTGGCTT |
Cldn 1 | AGATCCAGTGCAAGGTGTACG | CTGACAGACCTGCAATGATGAAG |
Cldn 10 | TCCAACTGCAAGGACTTCCC | GCACAGCCCACACAGTATGA |
CSF1R | AGCTCTCACCTGGAACCAAC | AGGCTTCTCTTGTCCTTCAACC |
Cxcl8 | GCTCTGTCGCAAGGTAGGAC | GGCCATAAGTGCCTTTACGA |
CX3CR1 | GGCTGTCTCGGACCTTCTTT | TTGCAGGGGATAGTGCCAAG |
CyclinA2 | CAAGCTCCAGAATGAAACTC | GATGTAGACGAACTCTGCTA |
CyclinB2 | GTTCTGTCTCCTGTCCCTAT | AGCTCAAGCTGTCTCAGATA |
Fabp2 | GCTGACGGGACTGAACTTTCA | CGCTGTGAGTACTTTTCCATTATCTTT |
Fabp6 | ACTATAGACAAGGAAGCAGACATGGA | TCCATTTTGACTGTTGCCTTGA |
FLT3 | TTTGGACAGGTTCTCGGCTC | CTCCCGCCTTGCTAATTCCA |
G10 | AACAGCCTCTGCATCCACAGT | TCAAGGAAGGGTACGCTGACA |
Gapdh | CCACAACATACTCAGCACCTGC | GTCCTCTGGCAAAGTCCAAG |
Jam2 | AGACAGGAACAGGCAGTGCT | TCCAATCCCATTTGAGGCTA |
Klf4 | CAAAGCCCAAGAGGGGACGG | GCAAACTTCCATCCGCAGCCT |
Krt7 | TGACAAAGGGCGTCTGGAGG | GAGCACCACGAACTCATTCTC |
Lgr5 | GCTGGCTTCTCTTCGTTCTCT | ATGTAGCCCCGTCACAGGAAA |
Lrig1 | GCATCGTGCTGACTTCGCTG | TAGTCTCATCTGTGTTGGTGAC |
Mmp7 | TACACACCTGACCTACCCCGA | GGCTGAAAAGCATGAGCTAATG |
MRC1L-B | CGTTCCGGTGGATAGATGGAAG | GGGAGCAATACTTGGACGAA |
Muc2 | TGGAGGCAAAGTGTCTGCTCT | TAATAGCATGGGCATTTGGAGAT |
Mx1 | GGGGAACCAGCCACAAGATA | TTAGTGAGGACCCCAAGCGT |
Myd88 | CGTGCCAAAGACTTCAGAGC | ACCATCCTCCGACACCTTCTT |
Nfkb1 | CGAACAGCAGATGGACCGTA | TTACCCACCAAGCTGTGAGC |
Occludin | CCGTAACCCCGAGTTGGAT | ATTGAGGCGGTCGTTGATG |
Olmf4 | CGACAGACGTGACTCCTCCTG | GGTGTGCTGGCTGGTAGTCTT |
Reg4 | GGAGGCAGAGGCACAGTGTC | CTCAGCGTGGCTGCTTCCTT |
Socs1 | CACGCACTTCCGAACCTTTC | ACTTCAGCTTCTCATGGGCG |
Stat1 | AAGCAAACGTAATCTTCAGGATAAC | TTTCTCTCCTCTTTCAGACAGTTG |
Stat3 | AAGGGTGACCCAATTGTCCA | TGTTAAACTTCCGGGACCCCC |
Tbp | AGCTCTGGGATAGTGCCACAG | ATAATAACAGCAGCAAAACGCTTG |
Tjp1 | ACCGAGAGATGCTGGTACTG | GCACAGCCTCATTCTCATGG |
Tjp2 | CATTGTTCGGGAGGATGCTG | AGCCAGCCAGTTTCCTAGTT |
Tjp3 | GGATACAGTGCGGCAGATTG | TGGTAGCAGTGAAGAGGTGG |
Tlr1 | TGAGCTTCATGACCAGCCGT | TGGTTGTTTTGTAGGTCCACT |
Tlr15 | AGCTGAACTGCTGCCACATTT | TTTCCTCTGTTCTTCTTTGTCTGAATC |
Tlr3 | GATCCATGGTGCAGGAAGTTT | CTGGCCAGTTCAAGATGCAG |
Tlr4 | ACCTCAATGCGATGCACTCT | AGTCCGTTCTGAAATGCCGT |
Tlr5 | TGTGTTGTGACCAGGCAGTT | AATCTTCAGGCCAACGCAGA |
Vil1 | GCAACTTGTGTCAGGGCTCACC | CCAGCACGTCCAGTGGGAAGGT |
Wdr43 | TCCCTATTCTAGCGGCTGCGT | GTTCACAACTGGCGTCCTCAC |