Figure 6

Impact of S. Typhimurium on immune host responses. The relative gene expression of specific genes involved in host response was analyzed by qRT-PCR in infected and non-infected organoids of caecum (black boxes) and ileum (white boxes). The values of gene expression were calculated with 2^−∆Ct for each sample. Results on histograms correspond to the mean ± SEM of the ratio of 2^−∆Ct (fold change) between infected and non-infected conditions and obtained from 2 independent experiments with n = 4 to 6 samples per experiment. A first statistical analysis was performed to compare 2^−∆Ct values of infected and non-infected (Mann–Whitney test, ***p < 0.001, **p < 0.01, *p < 0.05 on the top of boxes). The fold-change values of caecal organoids (Infected/non-infected) and ileal organoids were statistically compared (Mann–Whitney test, ^###p < 0.001, ^##p < 0.01, ^#p < 0.05)