Figure 5

Impact of S. Typhimurium on intestinal cell proliferation. The relative gene expression of specific genes involved in intestinal cell proliferation (Lgr5, Olmf4, CD44, CyclinA2, B2, CD24 and Lrig1) was analyzed by qRT-PCR in infected and non-infected organoids of caecum (black boxes) and ileum (white boxes). The values of gene expression were calculated with 2^−∆Ct for each sample. Results on histograms correspond to the mean ± SEM of the ratio of 2^−∆Ct (fold change) between infected and non-infected conditions and obtained from 2 independent experiments with n = 4–6 samples per experiment. A first statistical analysis was performed to compare 2^−∆Ct values of infected and non-infected (Mann–Whitney test, ***p < 0.001, **p < 0.01, *p < 0.05 on the top of boxes). The fold-change values of caecal organoids (Infected/non-infected) and ileal organoids were statistically compared (Mann–Whitney test, ^###p < 0.001, ^##p < 0.01, ^#p < 0.05)