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Figure 4 | Veterinary Research

Figure 4

From: Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken

Figure 4

Interaction of S. Typhimurium with the chicken intestinal epithelium. Organoids were infected with either MC1061 (MC1061) or S. Typhimurium (STm). Strains were grown in LB medium and then deposited onto organoids before gentamicin treatment. A Replication of S. Typhimurium in chicken intestinal organoids. The bacterial replication rate was determined at 24 hpi and the mean ± SEM obtained from at least two independent experiments with 4 infected wells per experimental condition are represented. B Invasiveness of S. Typhimurium in chicken organoids. The number of internalized bacteria was determined at 4 h 30 pi. Results are mean ± SEM obtained from at least two independent experiments with 4 infected wells per experimental condition. C Cell viability of uninfected and infected chicken organoids. At 24 hpi, the relative luminescence as indicator of viability was generated using Cell Titer-Glo 3D assay (**p-value < 0.01, *p-value < 0.05, ns: non-significant).

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Veterinary Research

ISSN: 1297-9716