Table 1 Real-time Q-PCR primers used for quantification of virus genomes and host cell gene.
Target | Primer sequence (5′–3′) |
|---|---|
IL-22 | F: CAGGACACTGACAACAGGCT |
R: CCACCTCCTCAGTGTATGGG | |
TRIM25 | F: GGAAAGCTAAGCCCCCTCAA |
R: TTGGTGCTCGGTTATCTGCC | |
IFN-α | F: TCCTCCAACACCTCTTCGAC R: GGGCTGTAGGTGTGGTTCTG |
IFN-λ | F: ACCAGGCTCTTCAATCGGAA R: TTGGGTGAAGAAGGCCAGG |
DHAV | F: AGACACATGTTGCTGAAAAACT R: TCCCCCTTATACTTAATGCCAG DHAV-1-Probe: Cy5-5′-ATGCCATGACACTAT CTCATATGAGTCAGC-3′-BHQ-2 |