Figure 5
TRIM25 highly inhibited DHAV-1 replication. A TRIM25 was downregulated via RNAi assay and the silencing effect was measured at 72 hpt. B TRIM25 was upregulated by transfecting pcDNA-TRIM25 into DEFs. TRIM25 expression was highly facilitated at 48 hpt. C DEFs cells in the siTRIM25 group, EXTRIM25 group, and control group were then infected with ~104.0 copies of DHAV-1 particles, and the viral growth curves for the different groups were obtained at 24 hpi via qRT-PCR. D TheDHAV-1 expression levels in the three groups were measured via Western blotting, anti-DHAV (1:100), and HRP-conjugated goat anti-mouse antibody (1:3000). E The grayscale values of DHAV-1 were measured. The results are expressed as the mean ± SEM of three independent experiments. ***P < 0.001.