Figure 3

IL-22 enhanced the IFN-λ-induced production of TRIM25. A Treating DEFs with IFN-λ highly facilitated TRIM25 expression. DEFs were divided into three groups which were each treated with PBS, and purified IFN-λ at 1 ng/mL or 10 ng/mL for 24 h; TRIM25 expression was then measured via qRT-PCR and Western blotting using anti-TRIM25 (1:100) and HRP-conjugated goat anti-mouse antibody (1:3000). The grayscale value of each band was measured using Image J software. B The DEFs in the three groups were separately inoculated with PBS, purified IL-22 at 10 ng/mL or 50 ng/mL and purified IFN-λ at 10 ng/mL for 24 h, and the TRIM25 expression levels were measured via qRT-PCR and Western blotting. C The DEFs in the three groups were separately transfected with PBS, pcDNA3.1 vector, and pcDNA-IL-22, and the overexpression effect was measured at 48 hpt. Subsequently, purified IFN-λ at 10 ng/mL was added into the DEFs to induce TRIM25 expression, which was measured 24 h later via qRT-PCR and Western blotting. D The DEFs in the three groups were separately inoculated with PBS, IL-22 neutralizing antibody at 10 ng/mL and 50 ng/mL, and purified IFN-λ at 10 ng/mL for 24 h, and the TRIM25 expression levels were measured via qRT-PCR and Western blotting. The results are expressed as the mean ± SEM of three independent experiments. *P < 0.05, ***P < 0.001, ns represents P > 0.05.