Figure 2
IL-22 inhibited DHAV-1 replication by inducing the production of high amounts of IFN-λ. A Experimental DEFs were treated with purified IL-22 at a concentration of 10 ng/mL and ~104.0 copies of DHAV-1, while those in the control group were treated with the same amount of PBS and DHAV-1 particles; the viral replication levels in both groups were measured 24 h later via qRT-PCR. B The viral copies in the control group were set as 100%, the percentage of viral copies in experimental group was then indicated. C The DHAV-1 expression level in both groups was measured through Western blotting using anti-DHAV (1:100) and HRP-conjugated goat anti-mouse antibody (1:3000). The grayscale value of each band was measured using Image J software. D DEFs contained in a 6-well plate were inoculated with ~104.0 copies of DHAV-1 or same amount of PBS; the expression of IFN-λ was measured via qRT-PCR. The results are expressed as the mean ± SEM of three independent experiments. **P < 0.01, ***P < 0.001.