Table 1 Cut-off points used for the IFN-γ ELISA assays
Test | Cut-off | Result | Sample |
|---|---|---|---|
IDvet | S/P ≥ 35% | Negative | S/P < 35% |
Positive | S/P ≥ 35% | ||
Non-valid | S/Pmitogen < 35% or ODPBS > 2.5 or | ||
ODbovis and ODavium > 2.5 | |||
Bovigam | ODbovis–ODPBS ≥ 0.1 and ODbovis–ODavium ≥ 0.1 | Negative | ODbovis–ODPBS < 0.1 or |
ODbovis–ODavium < 0.1 or | |||
ODbovis–ODPBS ≥ 0.1 and ODbovis–ODavium < 0.1 or | |||
ODbovis–ODPBS < 0.1 and ODbovis–ODavium ≥ 0.1 | |||
Positive | ODbovis–ODPBS ≥ 0.1 and ODbovis–ODavium ≥ 0.1 | ||
Non-valid | ODmitogen–ODPBS < 0.1 or ODPBS > 3.5 or | ||
ODbovis and ODavium > 3.5 |