Table 1 Primer sequences of the target genes.
Target | Sequence (5′–3′) | Orientation |
|---|---|---|
B646L | ATAGAGATACAGCTCTTCCG | Forward |
B646L | GTATGTAAGAGCTGCAGAC | Reverse |
GADPH | CCTTCCGTGTCCCTACTGCCAAC | Forward |
GADPH | GACGCCTGCTTCACCACCTTCT | Reverse |
TLR4 | TGTGCGTGTGAACACCAGAC | Forward |
TLR4 | AGGTGGCGTTCCTGAAACTC | Reverse |
MyD88 | GGCAGCTGGAACAGACCAA | Forward |
MyD88 | GGTGCCAGGCAGGACATC | Reverse |
IL-18 | CGATGAAGACCTGGAATCGG | Forward |
IL-18 | CATCATGTCCAGGAACACTTCTCTG | Reverse |
IL-1β | AAGAGGGACATGGAGAAGCGATTTG | Forward |
IL-1β | TTGTTCTGCTTGAGAGGTGCTGATG | Reverse |
IL-6 | TGCCGGCCTGCTGGATAAGC | Forward |
IL-6 | TGGCCCTCAGGCTGAACTGC | Reverse |
TNF-α | CCAATGGCAGAGTGGGTATG | Forward |
TNF-α | TGAAGAGGACCTGGGAGTAG | Reverse |
NLRP3 | CCTTCAGGCTGATTCAGGAG | Forward |
NLRP3 | GACTCTTGCCGCTATCCATC | Reverse |
ASC | GACAACAAACCAGCACTGCACTTCG | Forward |
ASC | ACTGCCTGGTACTGCTCTTCCGT | Reverse |
caspase-1 | ATCTCACCGCTTCGGACATGGCTAT | Forward |
caspase-1 | GTATTTCTTCCCACAAATGCCAGCC | Reverse |
GSDMD | ATGGCATCAGCCTTTG | Forward |
GSDMD | CTAGCAGAGCTGGCTG | Reverse |