Figure 6

DHM inhibited ASFV replication by inhibiting activation of the NLRP3 inflammasome. A Fluorescent images of ASFV-infected PAMs stained with DCFH-DA after a 24-h treatment with DHM. B–D Western blotting of PAMs were treated with different concentrations of EUK-134 or with DHM after ASFV infection; samples were collected after 24 h of treatment or at the indicated time points. E Western blotting of PAMs infected with 0.1, 1, or 10 MOI ASFV solution before DHM treatment; samples were collected after 24 h of treatment. F–G RT-qPCR of ASFV-infected PAMs treated with DHM for 24 h. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the respective viral control. ###P < 0.001 compared to the respective mock control.