Figure 5

DHM treatment downregulated ASFV-induced inflammatory mediators via the MAPK/NF-κB signaling pathway. A and B Western blotting of ASFV-infected PAMs treated with DHM; samples were collected at 24 h of treatment or at the indicated time points. C Western blotting of PAMs infected with 0.1, 1, or 10 MOI ASFV solution before adding DHM; samples were collected after 24 h of treatment. D–G RT-qPCR of ASFV-infected PAMs treated with DHM; samples were collected at 24 h of treatment or at the indicated time points. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the respective viral control. ###P < 0.001 < 0.001 compared to the respective mock control.