Figure 4

DHM inhibited ASFV replication by regulating the TLR4/MyD88 signaling pathway. A and B PAMs were incubated with ASFV solution (1 MOI) for 2 h before treatment with DHM. The samples were further incubated and collected at 24 h or at the indicated time points, and the samples were examined using Western blotting. C PAMs were infected with 0.1, 1, and 10 MOI ASFV solution for 2 h before treatment with DHM. Samples were collected after 24 h for Western blotting. D and E RT-qPCR of PAMs infected with ASFV solution before treatment with DHM; samples were collected 24 h after treatment. F Western blotting of ASFV-infected PAMs after a 24-h treatment with resatorvid. G Western blotting of PAMs transfected for 36 h with control siRNA (NC), siTLR4-a, or siTLR4-b. H PAMs were transfected with 50 nM siRNA control (NC), siTLR4-a, and siTLR4-b for 36 h. Next, the supernatants were removed, and PAMs were incubated with ASFV (1 MOI). After 24 h, the cells were collected and tested for Western blotting. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the respective virus control. ###P < 0.001 compared to the respective mock control.