Figure 2
Anti-ASFV effects of DHM. A Chemical structures of DHM. B The viability of DHM-treated PAMs. C–H The anti-ASFV activity of DHM was evaluated using C indirect immunofluorescence assay, D Western blotting, E, G hemadsorption HAD50, and F, H RT-qPCR. PAMs were attached to the cell plates and incubated with ASFV solution (1 MOI) for 2 h. The supernatant was removed, and cells were replaced with various concentrations of the DHM. The samples were further incubated and collected at 48 h or the indicated time. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the respective viral control.