Figure 2

CSFV-PCV2 bivalent vaccine provokes antigen-specific cellular immune response in SPF pigs. SPF pigs were immunized with two doses of CSFV-PCV2 bivalent vaccine (Group A, n = 4) or placebo (Group C, n = 4) at 6 and 8 weeks of age. PBMCs were isolated and stimulated with the viruses (CSFV LPC strain and PCV2) and subunit proteins (CSF-E2 and PCV2 capsid protein). The antigen-specific IFN-γ secreting cells for 1 MOI LPC virus (A), 10 µg of CSF-E2 subunit protein (B), 1 MOI PCV2 virus (C), and 10 µg of PCV2 capsid protein (D) were calculated. Data are presented in a box plot, and each spot indicates an individual mouse. The Kruskal–Wallis test was used for statistical analysis, and differences were considered statistically significant at a p value < 0.05.