Figure 1

CSFV-PCV2 bivalent vaccine provokes immune responses in a mice model. ICR mice were intraperitoneally immunized (0.5 mL/dose) with CSFV-PCV2 bivalent vaccine (n = 5) or placebo (n = 5) at 6 and 8 weeks of age. Serum samples were collected at 12 weeks of age to evaluate CSFV-specific (A) or PCV2-specific (B) antibody levels. Splenocytes were isolated at 14 weeks of age to evaluate the number of CSFV-specific (C) and PCV2-specific (D) IFN-γ-secreting cells by ELISpot assay. Data are presented in a box plot, and each spot indicates an individual mouse. The Kruskal–Wallis test was used for statistical analysis, and differences were considered statistically significant at a p value < 0.05.