Improving the consistency of experimental swine dysentery inoculation strategies

Table 1 Methodological details for six swine dysentery inoculation experiments

Trial				Animal		Inoculum
Id	Length (dpi)	Acclimation period (days)	Pig source (Pig age)	Diet	Housing and inoculation route	Volume Specie/strain State	Total dose (GE × 10¹¹)
A	15	10	Prairie Swine Centre (6 wks)	12977	Individual IG	100 mL Bhyo D19 Frozen/thawed	10.4
A	15	10	Prairie Swine Centre (6 wks)	12977	Group housed IG	100 mL Bhyo D19 Frozen/thawed	10.4
B	21	14	Prairie Swine Centre (7 wks)	12977	Individual IG	50 mL Bhyo D19 Fresh	4.92
B	17	14	Prairie Swine Centre (7 wks)	12977	Individual IG	50 mL Bhyo G44 Fresh	0.51
C	19	11	Last Mountain nursery farm (7 wks)	JH002 grower	Individual IG	50 mL Bhamp 30446 Fresh	0.25
	19	11				100 mL Bhamp 30446 Fresh	0.51
	20	14				50 mL Bhyo G44 Fresh	6.87
	20	14				100 mL Bhyo G44 Fresh	13.7
D	14	9	Prairie Swine Centre (7 wks)	12977	Individual IG	100 mL Bhyo G44 Fresh	14.8
D	14	9	Prairie Swine Centre (7 wks)	12977	Individual Feedballs	100 mL Bhyo G44 Fresh	14.8
E	15	14	Olymel-Krysa nursery farm (5 wks)	12977	Individual IG	100 mL Bhyo G44 Fresh	3.15
E	15	14	Olymel-Krysa nursery farm (5 wks)	12977	Individual Oral-syringe 100 mL	100 mL Bhyo G44 Fresh	3.15
F	13	14	Last Mountain nursery farm (7 wks)	JH002 grower	Individual IG	100 mL Bhyo G44 Fresh	4.26
F	13	14	Last Mountain nursery farm (7 wks)	JH002 grower	Individual Oral-syringe 300 mL	300 mL Bhyo G44 Fresh	4.26

dpi: days post-inoculation, wks: weeks of age, 12977/JCH002: diet names, IG: intragastric, Bhyo: Brachyspira hyodysenteriae (strains G44 or D19), Bhamp: B. hampsonii (strain 30446), GE: genomic equivalents.
Total dose = average inoculum concentration (per day) × volume administered × number of inoculation days.

ISSN: 1297-9716