Figure 4

Principal coordinate analysis plots based on Bray-Curtis distances on generated microbiota data. In panel A coloring is based on individual cows, in panel B coloring is based on time in relation to infusion for control cows, in panel C time in relation to infusion for LPS-infused cows. In both panels B and C, blue color scale represents samples collected before infusion, green color scale represents samples collected 1–4 h post-infusion and the red color scale represents samples collected > 23 h post-infusion. Panel D is colored according to the amount of bacterial growth on plates (0 cfu (black colour), 1–10 cfu (yellow colour), 11–50 cfu (green colour) or > 50 cfu (red colour) per 100 µL of cultured milk after 48 h). In panel E grouping is based on SCC (< 150 000 (yellow colour), 150 000–1 000 000 (green colour) or > 1 000 000 (red colour) cells per mL) and in panel F all samples are included where green dots represents control cows, red dots; LPS infused cows, blue dots; negative controls (NTC and negPCR) and black dots mock communities.