Table 1 Sequences of DNA primers.
Name | Primer sequence (5′-3′) |
|---|---|
| Â | Primers for CDS Cloning |
MKP1-CDS-F | CCCTCGAGATGGTGAACCTGCGGGTGT |
MKP1-CDS-R | TTGCGGCCGCTCAGCAGCTCGGGGAGG |
|  | Primers for 3’UTR Cloning |
MKP1 3’UTR-F | CCCTCGAGTTGTCCTAAGCCTGCTGCAT |
MKP1 3’UTR-R | TTGCGGCCGCGCAACTCAAGAAGCACTCGC |
Mut- MKP1 3’UTR-F | AGAGAAACCAAATGATCGAGTATTTTTTTTTTGGTACTGTAATCCTGTGTGT |
Mut- MKP1 3’UTR-R | AAATACTCGATCATTTGGTTTCTCTAGAAATGCAACTTCAGGTTCATG |
|  | Primers for RT‒qPCR |
GAPDH-F | GAGGGTAGTGAAGGCTGCTG |
GAPDH-R | CACAACACGGTTGCTGTATC |
MKP1-F | GGCACTACCAGTACAAAAGCATC |
MKP1-R | CAAGGACCTGCGACTCGAAC |
ERK-F | GTGACTTCGGACTGGC |
ERK-R | TCCTGGAAAGATGGGT |
P38-F | ACGGCTACTGAAGCATAT |
P38-R | TCTGTAAGTTTCTGGCATT |
JNK-F | AAGCAAGCGTGACAAC |
JNK-R | ATCATAGGCTGCACATAC |
C-jun-F | CGGCTCATCATCCAGTCC |
C-jun-R | CCCTCTTGCTCGTCGGTA |
C-fos-F | TACACCTCCACCTTCGTCTTCACC |
C-fos-R | TTGCTGCTGCTGCCCTTCC |
CCL4-F | CCTCGCTGTCCTCCTCA |
CCL4-R | CTGGCTGTTGGTCTCGTAG |
CCL5-F | CTCAAGCCTCTTCATCTCC |
CCL5-R | GCATTTGCTGCTGGTGT |
PMGA1.2-F | TCATATAAAAACTTAATGGCTACTC |
PMGA1.2-R | CTCTTCTGTGATTGTATTAGCAG |
RT-Let-7d-F | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACTATGCA |
miR-let-7d-F | CTGGTAGGAGAGGTAGTGGGTTGC |
miR-let-7d-R | ACTGGTGTCGTGGAGTCGGC |
5s-rRNA-F | CCATACCACCCTGGAAACGC |
5s-rRNA-R | TACTAACCGAGCCCGACCCT |
RT-5Â S | AACTGGTGTCGTGGAGTCGGC |