Figure 8
The effect of let-7d on CP-II cell proliferation and apoptosis. CP-II cells were transfected with let-7d 50 nM mimics, 100 nM inhibitors, or their respective controls and infected with 100 µL of MG-HS (1 × 109 CCU/mL). Then, a CCK-8 kit was used to detect cell proliferation at different times (A). After 48 h of treatment, the CP-II cells were stained with Annexin V– PI and analysed by flow cytometry. The apoptotic cell ratio is shown in (B). All data from the experiments carried out independently three times are shown as the mean value ± SD, and * p < 0.05, ** p < 0.01 indicated significant differences.