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Figure 4 | Veterinary Research

Figure 4

From: Low let-7d microRNA levels in chick embryos enhance innate immunity against Mycoplasma gallisepticum by suppressing the mitogen-activated protein kinase pathway

Figure 4

Let-7d activates the MAPK pathway. A, C Relative mRNA expression of p38, ERK, jun and JNK. GAPDH was used as the internal quantitative control gene. β-actin and GAPDH served as the loading controls. B Effects of gga-let-7d on MAPK pathway downstream related genes in CP-II. RT‒qPCR was used to detect the relative expression of mRNA. GAPDH was used as the internal quantitative control gene. The data are presented as the means ± SDs. One-way ANOVA was used to analyse significant differences (different lowercase letters represent p < 0.05).

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