Figure 1

Relative let-7d expression level in CP-II cells and chicken embryonic lung tissue. (A) The relative level of let-7d in chicken embryonic lungs. (B) The relative level of let-7d in CP-II cells. The above data were corrected via 5sRNA as the internal quantitative control gene. (C, D) qPCR and WB were used to detect the mRNA or protein expression of pMGA1.2. Positive chicken serum was used as the primary antibody against pMGA1.2. (E) Downregulated let-7d regulates inflammatory cytokine production in CP-II cells. The levels of IL-6, IL-1β, IL-10 and TNF-α were analysed by ELISAs at 48 h post-transfection. All measurements shown are the mean ± SD from three independent experiments, each with three replicates. (*p < 0.05, **p < 0.01). Different lowercase letters represent p < 0.05.