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Table 3 Antibody response of water buffaloes immunized against BoHV-1 using an immunisation protocol with gE-deletion marker vaccines and challenge infected with wild-type BuHV-1

From: Endogenous and viral microRNAs in nasal secretions of water buffaloes (Bubalus bubalis) after Bubaline alphaherpesvirus 1 (BuHV-1) challenge infection

Group

 

Post-challenge day (pcd)

0*

2

4

7

10

15

30

63

A

gE-ELISAa

 + 

 + 

gB-ELISAb

 + 

 + 

 + 

 + 

 + 

 + 

 + 

 + 

ELISAc

 + ^

 + ^

B

gE-ELISAa

 + 

 + 

gB-ELISAb

 + 

 + 

 + 

 + 

ELISAc

 + ^

 + ^

 + ^

  1. *The day of challenge corresponding to 270 pvd; A, Vaccinated group; B, Control group.
  2. aIDEXX IBR gE Ab test, MAINE, USA. This test detects antibodies to glycoprotein E (gE) of BoHV-1/BuHV-1.
  3. bIDEXX IBR gB X3 Ab, Maine, USA. This test detects antibodies to glycoprotein B (gB) of BoHV-1/BuHV-1.
  4. cIN3Diagnostic Eradikit™ BoHV-1-BuHV-1 discrimination kit, Turin, Italy. This test discriminates antibodies to glycoprotein E (gE) against BoHV-1 and BuHV-1. NA, + ^, positive to BuHV-1.