Figure 3
DTMUV N7-MTase mutants induce cellular and humoral immune responses in ducklings. A Scheme of immunization. 5 day-old SPF ducklings were intramuscularly vaccinated with a 200 µL volume containing either 104.75FFU/mL DTMUV WT, K61A, K182A, E218A, WF100 vaccine strain or DMEM mock. At 3 weeks post-immunization (wpi), serum samples of infected ducklings were collected for detection of IFN-γ and IL-4 by ELISA. B IFN-γ levels in serum. Each of these was conducted with three parallel replicates. Bars show means ± SDs. **P < 0.01; *P < 0.05 (one-way ANOVA). C IL-4 levels in serum. Each of these was conducted with three parallel replicates. Bars show means ± SDs. ***P < 0.001; **P < 0.01; *P < 0.05 (one-way ANOVA). D Serum anti-DTMUV IgG antibodies were measured by ELISA at 2, 3, 4, and 6 wpi. Each of these was conducted with three parallel replicates. Bars show means ± SDs. ***P < 0.001; **P < 0.01; *P < 0.05; ns, no significance, P > 0.05 (two-way ANOVA).