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Table 1 Peptides used as antigens

From: Proof of concept for multiplex detection of antibodies against Chlamydia species in chicken serum using a bead-based suspension array with peptides as antigens

Species

Peptide name

Descriptiona

Sequence

Length

Source

C.gallinacea

pCga001

Cga_08DC63_OmpA_326-345

NPSFLGSADAQATLVDSVQI

20

[15, 17]

 

pCga002

Cga_08-1274/3_IncA_297-326

SEAATSTSTPEGETSETKEGEEDSSVVEFD

30

[15, 16]

 

pCga003

Cga_08DC63_IncA_303-326

TSTPEGETSETKEGEEDSSVVEFD

24

[17]

 

pCga008

C. gal G47_OmpA

NPTFSGGAVPQTGGTGSVVDVVQI

24

This study

 

pCga009

C. gal F725_OmpA

NPSFLGEANAQAKLVDSVQI

20

This study

 

pCga010

C. gal G47_IncA

SEAATSTSTPEGETSETKEGEEDSSVIEFD

30

This study

 

pCga011

C. gal F725_IncA

SEAATSTSTPEGETSETKEGEEDSSVIDFD

30

This study

C. psittaci

pCps001

Cps_02DC15_OmpA_158-181

LVGLIGFSAASSISTDLPTQLPNV

24

[15,16,17]

 

pCps002

Cps_02DC15_IncA_329-352

ADQGDLRDPSGDRYGGWGAQSSYR

24

[17]

 

pCps009

Cps_02DC15_OmpA_250-265

ASSNFPLPITAGTTEA

16

[16]

 

pCps010

Cps_02DC15_OmpA_329-352

SLIGSTTALPNNSGKDVLSDVLQI

24

[17]

 

pCps013

Cps_02DC15_PmpD_1053-92

DPNAKPAEKIESPTSKVYYSAYDPVKNPGKKTLADINSIG

40

[16]

 

pCps014

Cps_02DC15_IncA_321-360

SLTSTTETADQGDLRDPSGDRYGGWGAQSSYRLSPSVTMS

40

[15, 16]

 

pCps015

Cps_02DC15_CT618_105-134

YEVDSATGSFKIVTKNIQKPNGEVEIVSSR

30

[15, 16]

 

pCps016

Cps_02DC15_CT618_189-228

CGAVDDVISIVSTLRSTDFDPSYEDLVQRRVTLREKFFSL

40

[15, 16]

C.abortus

pCab001

Cab_S26/3_OmpA_89-104

PTGTAAANYKTPTDRP

16

[16]

 

pCab002

Cab_S26/3_OmpA_153-176

NLVGLIGVKGSSIAADQLPNVGIT

24

[15,16,17]

 

pCab003

Cab_S26/3_OmpA_158-173

GVKGSSIAADQLPNV

15

[17]

 

pCab004

Cab_S26/3_PmpD_1064-1087

PTSNVYYSAHESVKQPENKTLADI

24

[17]

 

pCab005

Cab_S26/3_IncA_324-353

STAVTEHADIPRDPNRDPRGGRGGQSSPSV

30

[15, 17]

C. avium

pCav001

Cav_10DC88_IncA_305-328

ESTPVEAPESKEEAKDTAEVAAEG

24

[17]

 

pCav002

Cav_10DC88_IncA_299-328

TVEGAAESTPVEAPESKEEAKDTAEVAAEG

30

[15]

 

pCav003

Cav_10DC88_IncA_325-348

AAEGSGSTEESKGKEDDKSGDKKE

24

[17]

 

pCav004

Cav_10DC88_IncA_319-348

KDTAEVAAEGSGSTEESKGKEDDKSGDKKE

30

[15, 16]

n.a

NC

Biotin-spacer-(no peptide)-amide

n.a

0

this study

  1. Peptide sequences were selected for four Chlamydia species based on literature and on sequences of Dutch isolates G47 and F725. Partial alignments can be found in Tables 2 and 3.
  2. P: purified peptide; Cga, Cps, Cab, and Cav refer to the Chlamydia species (see first column); NC: negative control; n.a.: not applicable.
  3. aC. gallinacea strain 08DC63 and 08-1274/3 are the same.
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Veterinary Research

ISSN: 1297-9716